Development of a real-time PCR method for the simultaneous, detection of mackerel and horse mackerel
Prado, M.; Boix, A.; von Holst, C. (2013). Development of a real-time PCR method for the simultaneous, detection of mackerel and horse mackerel. Food Control 34(1): 19-23. dx.doi.org/10.1016/j.foodcont.2013.04.007
In: Food Control. Butterworth Scientific/Elsevier: London. ISSN 0956-7135; e-ISSN 1873-7129, more
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| Keywords |
Scomber scombrus Linnaeus, 1758 [WoRMS]; Trachurus trachurus (Linnaeus, 1758) [WoRMS]
Marine/Coastal |
| Author keywords |
Real-time PCR; Nuclear DNA; Fish; DNA quantification; Parvalbumin gene;Food authenticity |
| Abstract |
We have developed a real-time PCR method for the simultaneous detection of horse mackerel (Trachurus trachurus) and mackerel (Scomber scombrus) DNA. This method has been designed to be used as an extension to our previously published method for the simultaneous detection of several fish species. A fragment of the parvalbumin gene was used as target for the development of the reported method. The results showed the required specificity, efficiency of the real-time PCR reaction and comparable selectivity with the previously reported method, therefore their possible complementarity for food and feed analysis is discussed. Additionally, the developed method combined with melt curve analysis allows the differentiation between both fish species which can have interesting applications for authenticity studies. |
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