Paludisphaera borealis gen. nov., sp. nov., a hydrolytic planctomycete from northern wetlands, and proposal of Isosphaeraceae fam. nov.
Kulichevskaya, I.S.; Ivanova, A.; Suzina, N.E.; Rijpstra, W.I.C.; Sinninghe Damsté, J.S.; Dedysh, S.N. (2016). Paludisphaera borealis gen. nov., sp. nov., a hydrolytic planctomycete from northern wetlands, and proposal of Isosphaeraceae fam. nov. Int. J. Syst. Evol. Microbiol. 66: 837-844. dx.doi.org/ 10.1099/ijsem.0.000799
In: International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology: Reading. ISSN 1466-5026; e-ISSN 1466-5034, more
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| Authors | | Top |
- Kulichevskaya, I.S.
- Ivanova, A.
- Suzina, N.E.
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- Rijpstra, W.I.C., more
- Sinninghe Damsté, J.S., more
- Dedysh, S.N.
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| Abstract |
Two isolates of aerobic, budding, pink-pigmented bacteria, designated strains PX4T and PT1, were isolated from a boreal Sphagnum peat bog and a forested tundra wetland. Cells of these strains were non-motile spheres that occurred singly or in short chains. Novel isolates were capable of growth at pH values between 3.5 and 6.5 (optimum at pH?5.0–5.5) and at temperatures between 6 and 30?°C (optimum at 15–25?°C). Most sugars and a number of polysaccharides including pectin, xylan, lichenin and Phytagel were used as growth substrates. The major fatty acids were C16?:?0, C18?:?1?9 and C18?:?0; the major polar lipids were phosphocholine and trimethylornithine. The quinone was menaquinone-6, and the G+C content of the DNA was 66?mol%. Strains PX4T and PT1 were members of the order Planctomycetales and displayed 93–94?% 16S rRNA gene sequence similarity to Aquisphaera giovannonii, 91–92?% to species of the genus Singulisphaera and 90–91?% to Isosphaera pallida. The two novel strains, however, differed from members of these genera by cell morphology, substrate utilization pattern and a number of physiological characteristics. Based on these data, the novel isolates should be considered as representing a novel genus and species of planctomycetes, for which the name Paludisphaera borealis gen. nov., sp. nov., is proposed. The type strain is PX4T (?=?DSM 28747T?=?VKM B-2904T). We also suggest the establishment of a novel family, Isosphaeraceae fam. nov., to accommodate stalk-free planctomycetes with spherical cells, which can be assembled in short chains, long filaments or shapeless aggregates. This family includes the genera Isosphaera, Aquisphaera, Singulisphaera and Paludisphaera |
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