A method for the specific detection of resident bacteria in brine shrimp larvae

Niu, Y.; Defoirdt, T.; Rekecki, A.; De Schryver, P.; Van Den Broeck, W.; Dong, S.; Sorgeloos, P.; Boon, N.; Bossier, P.

doi:/10.1016/j.mimet.2012.02.004

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A method for the specific detection of resident bacteria in brine shrimp larvae

Niu, Y.; Defoirdt, T.; Rekecki, A.; De Schryver, P.; Van Den Broeck, W.; Dong, S.; Sorgeloos, P.; Boon, N.; Bossier, P. (2012). A method for the specific detection of resident bacteria in brine shrimp larvae. J. microbiol. methods 89(1): 33-37. https://dx.doi.org/10.1016/j.mimet.2012.02.004

In: Journal of Microbiological Methods. Elsevier: Amsterdam. ISSN 0167-7012; e-ISSN 1872-8359, more

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Keyword

Artemia franciscana Kellog, 1906 [WoRMS]

Author keywords

Resident bacteria; Brine shrimp; DGGE; Intestinal bacteria

Authors		Top
Niu, Y., more Defoirdt, T., more Rekecki, A., more	De Schryver, P., more Van Den Broeck, W., more Dong, S.	Sorgeloos, P., more Boon, N., more Bossier, P., more

Abstract

In this study, we describe an easy but efficient method to specifically target the intestinal resident microbiota in brine shrimp larvae during DGGE analysis, hereby excluding the interference of both transient (luminal) bacteria and body surface bacteria. This effective technique has several advantages over alternative methods, with respect of ease of use and rapidity.

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