Isolation and characterisation of 14 novel microsatellite markers through Next Generation Sequencing for the commercial Atlantic seabob shrimp Xiphopenaeus kroyeri
Kerkhove, T.; Hellemans, B.; De Troch, M.; De Backer, A.; Volckaert, F.A.M. (2019). Isolation and characterisation of 14 novel microsatellite markers through Next Generation Sequencing for the commercial Atlantic seabob shrimp Xiphopenaeus kroyeri. Molecular biology reports 46(6): 6565-6569. https://dx.doi.org/10.1007/s11033-019-05026-9
In: Molecular biology reports. Springer. ISSN 0301-4851; e-ISSN 1573-4978, more
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| Author keywords |
Population structure; Shotgun sequencing; DNA microsatellites; Sustainable fisheries; Western Atlantic |
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- De Backer, A., more
- Volckaert, F.A.M., more
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| Abstract |
Assessing population genetic structure is a crucial step to support fisheries and conservation management. DNA microsatellite molecular markers are a widely used tool in population genotyping. In the present study, we characterised and developed 14 novel polymorphic microsatellite markers for a decapod crustacean, the Atlantic seabob shrimp Xiphopenaeus kroyeri (Heller, 1862), through rapid and cost-effective Illumina shotgun sequencing and a Galaxy-based bioinformatic pipeline. We genotyped 60 individuals from 2 populations with the newly developed microsatellites, resulting in the detection of 3 to 29 alleles per locus. Four loci deviated from Hardy–Weinberg equilibrium. Cross-amplification in a cryptic congeneric species was successful for eight loci (57%). The microsatellite loci developed in this study will be highly relevant for genetic and evolutionary studies of X. kroyeri, and for the stock management of this commercially exploited species. |
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