Fatty acid bioconversion in harpacticoid copepods in a changing environment: a transcriptomic approach
Boyen, J.; Fink, P.; Mensens, C.; Hablützel, P.I.; De Troch, M. (2020). Fatty acid bioconversion in harpacticoid copepods in a changing environment: a transcriptomic approach. Phil. Trans. R. Soc. Lond. (B Biol. Sci.) 375(1804): 20190645. https://dx.doi.org/10.1098/rstb.2019.0645
In: Philosophical Transactions of the Royal Society of London. Series B, Biological sciences. Royal Society: London. ISSN 0962-8436; e-ISSN 1471-2970, more
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harpacticoid copepods, fatty acid metabolism, transcriptomics, global warming |
| Authors | | Top |
- Boyen, J., more
- Fink, P.
- Mensens, C., more
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- Hablützel, P.I., more
- De Troch, M., more
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| Abstract |
By 2100, global warming is predicted to significantly reduce the capacity of marine primary producers for long-chain polyunsaturated fatty acid (LC-PUFA) synthesis. Primary consumers such as harpacticoid copepods (Crustacea) might mitigate the resulting adverse effects on the food web by increased LC-PUFA bioconversion. Here, we present a high-quality de novo transcriptome assembly of the copepod Platychelipus littoralis, exposed to changes in both temperature (+3°C) and dietary LC-PUFA availability. Using this transcriptome, we detected multiple transcripts putatively coding for LC-PUFA-bioconverting front-end fatty acid (FA) desaturases and elongases, and performed phylogenetic analyses to identify their relationship with sequences of other (crustacean) taxa. While temperature affected the absolute FA concentrations in copepods, LC-PUFA levels remained unaltered even when copepods were fed an LC-PUFA-deficient diet. While this suggests plasticity of LC-PUFA bioconversion within P. littoralis, none of the putative front-end desaturase or elongase transcripts was differentially expressed under the applied treatments. Nevertheless, the transcriptome presented here provides a sound basis for future ecophysiological research on harpacticoid copepods. This article is part of the theme issue ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids'. |
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