Production of intestine-specific monoclonal antibody and interspecific cross-reaction in triclads and polyclads
Asano, Y.; Yoshida, A.; Isozaki, N.; Ishida, S. (2001). Production of intestine-specific monoclonal antibody and interspecific cross-reaction in triclads and polyclads. Belg. J. Zool. 131(Suppl. 1): 137-141
In: Belgian Journal of Zoology. Koninklijke Belgische Vereniging voor Dierkunde = Société royale zoologique de Belgique: Gent. ISSN 0777-6276; e-ISSN 2295-0451, more
Also appears in:Saló, E.; Watson, N.; Schockaert, E. (Ed.) (2001). Proceedings of the 9 th International Symposium on the Biology of the Turbellaria, Barcelona, Spain, June 2000. Belgian Journal of Zoology, 131(Suppl. 1). Koninklijke Belgische Vereniging voor Dierkunde = Société royale zoologique de Belgique: Diepenbeek. 236 pp., more
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| Keywords |
Platyhelminthes [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Asano, Y.
- Yoshida, A.
- Isozaki, N.
- Ishida, S.
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| Abstract |
We have produced a monoclonal antibody (mAb(2)2B6) specific to intestinal and epidermal cells of Dugesia japonica. This mAb(2)2B6 also reacted with the secretory granules of a kind of sub-epidermal gland cells, epithelial cells and gland cells in the pharynx. The results of the interspecific cross-reaction tests with four other freshwater triclads and four polyclads showed that this mAb(2)2B6 can be used as a marker, specific to only intestinal cells in both Planocera multitentaculata and Planocera reticulata of polyclads. Immuno-electron microscopical research revealed that the cytoplasm of phagocytic cells and the spherical large granules in granular clubs reacted strongly to this mAb in the intestine. Also, mAb positive material seemed to be absorbed by pinocytosis from phagocytic cells into the granular clubs. These observations support the theory that the granular clubs are protein reserve cells, not gland cells for intraluminal digestion. |
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