Actinocrinis puniceicyclus gen. nov., sp nov., an actinobacterium isolated from an acidic spring
Kim, J.J.; Marjerrison, C.E.; Cornish Shartau, S.L.; Brady, A.L.; Sharp, C.E.; Rijpstra, W.I.C.; Sinninghe Damsté, J.S.; Schumann, P.; Grasby, S.E.; Dunfield, P.F. (2017). Actinocrinis puniceicyclus gen. nov., sp nov., an actinobacterium isolated from an acidic spring. Int. J. Syst. Evol. Microbiol. 67(3): 602-609. https://dx.doi.org/10.1099/ijsem.0.001667
In: International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology: Reading. ISSN 1466-5026; e-ISSN 1466-5034, more
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| Keywords |
Actinobacteria [WoRMS]; Actinocrinis puniceicyclus
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| Author keywords |
Actinocrinis puniceicyclus; acidophile; actinobacteria |
| Authors | | Top |
- Kim, J.J.
- Marjerrison, C.E.
- Cornish Shartau, S.L.
- Brady, A.L.
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- Sharp, C.E.
- Rijpstra, W.I.C., more
- Sinninghe Damsté, J.S., more
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- Schumann, P.
- Grasby, S.E.
- Dunfield, P.F.
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| Abstract |
An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada.Cells of isolate OB1T were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments. The pH range for growthwas pH 3.5–6.5 (optimum pH 5.5), and the temperature range was 13–30?C. The major cellular fatty acids were i-C16 : 0(28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose. The major quinone wasmenaquinone-11 (MK-11), and the peptidoglycan type was A1g. The DNA G+C content was 70.2 %. Along with growth oncomplex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on monoanddisaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).Anaerobic growth was not observed. The cells did not fix atmospheric nitrogen. On the basis of comparative 16S rRNA genesequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the orderActinomycetales. The most closely related species was Actinospica robiniae. However, the 16S rRNA gene sequence identity tothis bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species. We thereforepropose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp. nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain |
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