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Investigating sperm cryopreservation in a model tunicate, Ciona intestinalis sp. A
Sorrenti, G.; Bagnoli, A.; Miraglia, V.; Crocetta, F.; Vitiello, V.; Ristoratore, F.; Cirino, P.; Sansone, G.; Sordino, P. (2014). Investigating sperm cryopreservation in a model tunicate, Ciona intestinalis sp. A. Cryobiology 68(1): 43-49. https://dx.doi.org/10.1016/j.cryobiol.2013.11.005
In: Cryobiology. Academic Press: San Diego. ISSN 0011-2240; e-ISSN 1090-2392, more
Peer reviewed article  

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Keywords
    Exploitable Scientific Result
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    Marine Sciences
    Maritime Industries > Blue Biotech
    Policy Makers / Decision Makers
    Scientific Community
    Scientific Publication
    Marine/Coastal
Author keywords
    Ciona intestinalis; Spermatozoon; Cryopreservation; Dimethyl sulfoxide;Fertilization success

Project Top | Authors 
  • Association of European marine biological laboratories, more

Authors  Top 
  • Sorrenti, G.
  • Bagnoli, A.
  • Miraglia, V.
  • Crocetta, F., more
  • Vitiello, V.
  • Ristoratore, F.
  • Cirino, P.
  • Sansone, G.
  • Sordino, P.

Abstract
    In cryopreservation procedures, the capacity to protect the cells from freezing and thawing processes is sensitive to the choice of the cryoprotective agent (CPA) and to its optimal concentration. The advancement of research on Tunicate model species has raised interest in liquid nitrogen cryopreservation for the storage and distribution of genetic resources. Ciona intestinalis (Linnè, 1767) consists of a complex of cryptic taxa that are central to several areas of investigation, from comparative genomics to invasive biology. Here we investigated how five CPAs, three chilling rates and two freezing rates influence semen cryopreservation in C. intestinalis sp. A. By using larval morphology and motility as endpoints, we estimated that long term semen storage requires 10% dimethyl sulfoxide as a protective agent, −1 °C/min chilling rate (18 °C to 5 °C) and −13 °C/min freezing rate (5 °C to −80 °C), followed by immersion in liquid nitrogen.

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