An injectable, self-healing, 3D printable, double network co-enzymatically crosslinked hydrogel using marine poly- and oligo-saccharides for wound healing application
Jafari, H.; Alimoradi, H.; Delporte, C.; Bernaerts, K.V.; Heidari, R.; Podstawczyk, D.; Niknezhad, S.V.; Shavandi, A. (2022). An injectable, self-healing, 3D printable, double network co-enzymatically crosslinked hydrogel using marine poly- and oligo-saccharides for wound healing application. Applied Materials Today 29: 101581. https://dx.doi.org/10.1016/j.apmt.2022.101581
In: Applied Materials Today. ELSEVIER SCIENCE BV: Amsterdam. ISSN 2352-9407, more
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| Keyword |
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| Author keywords |
Marine polysaccharides; Injectable hydrogels; Glucose oxidize; Enzyme mediated crosslinking; Wound healing |
| Authors | | Top |
- Jafari, H., more
- Alimoradi, H.
- Delporte, C.
- Bernaerts, K.V.
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- Heidari, R.
- Podstawczyk, D.
- Niknezhad, S.V.
- Shavandi, A., more
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| Abstract |
In this study, we designed dual network hydrogels with antioxidant and antibacterial activities using marine poly- and oligosaccharides with skin wound healing potential. The synergy between dual enzymatic co-crosslinking based on glucose oxidize (GOx)/horseradish peroxidase (HRP) and electrostatic interaction between positively charged chitooligosaccharides (COS) and phenolated chitosan with negatively charged phenolated alginate formed a hydrogel. The Gel-COS hydrogels exhibited toughness, self-healing, moldability, injectability, and 3D printability. Investigation of the physicochemical properties of the hydrogels exhibited a swelling ratio (< 50%) and in vitro biodegradation after 9 days. Furthermore, the hydrogels exhibited antioxidant properties and antibacterial activity against E. coli and S. aureus. The hydrogels were not cytotoxic and enhanced the migration of 3D cell encapsulated 3T3-L1 fibroblasts, blood vessel formation, as well as in vivo wound healing in a rat model. The Gel-COS hydrogel can be considered a promising skin wound dressing material. |
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