| one publication added to basket [223221] | Bacterial host interaction of GFP-labelled Vibrio anguillarum HI-610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae
Rekecki, A.; Gunasekara, R.A.Y.S.A.; Dierckens, K.; Laureau, S.; Boon, N.; Favoreel, H.; Cornelissen, M.; Sorgeloos, P.; Ducatelle, R.; Bossier, P.; Van Den Broeck, W. (2012). Bacterial host interaction of GFP-labelled Vibrio anguillarum HI-610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae. J. Fish Dis. 35(4): 265-273. http://dx.doi.org/10.1111/j.1365-2761.2011.01342.x
In: Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne. ISSN 0140-7775; e-ISSN 1365-2761, more
|  |
| Keyword |
|
| Author keywords |
GFP-labelled pathogen; gnotobiotic model system; larviculture; microscopy; ultrastructure |
| Authors | | Top |
- Rekecki, A., more
- Gunasekara, R.A.Y.S.A., more
- Dierckens, K., more
- Laureau, S.
|
- Boon, N., more
- Favoreel, H., more
- Cornelissen, M.
- Sorgeloos, P., more
|
- Ducatelle, R., more
- Bossier, P., more
- Van Den Broeck, W., more
|
| Abstract |
The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V. anguillarum with sea bass larvae. In the present study, germ-free sea bass larvae were orally exposed to a V. anguillarum HI-610 pathogen labelled with the green fluorescent protein (GFP-HI-610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post-exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid- and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence. |
|
