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Mutation studies in ascidians: a review
Crocetta, F.; Marino, R.; Cirino, P.; Macina, A.; Staiano, L.; Esposito, R.; Pezzotti, M.R.; Racioppi, C.; Toscano, F.; De Felice, E.; Locascio, A.; Ristoratore, F.; Spagnuolo, A.; Zanetti, L.; Branno, M.; Sordino, P. (2015). Mutation studies in ascidians: a review. Genesis 53(1): 160-169. https://dx.doi.org/10.1002/dvg.22837
In: Genesis. Wiley-Blackwell: Hoboken. ISSN 1526-954X; e-ISSN 1526-968X, more
Peer reviewed article  

Available in  Authors 

Keywords
    Book/Review
    Marine Sciences
    Marine Sciences > Marine Genomics
    Marine Sciences > Marine Sciences General
    Scientific Community
    Scientific Publication
    Marine/Coastal
Author keywords
    mutations; genetics; ascidians; Ciona

Project Top | Authors 
  • Association of European marine biological laboratories, more

Authors  Top 
  • Crocetta, F., more
  • Marino, R.
  • Cirino, P.
  • Macina, A.
  • Staiano, L.
  • Esposito, R.
  • Pezzotti, M.R.
  • Racioppi, C.
  • Toscano, F.
  • De Felice, E.
  • Locascio, A.
  • Ristoratore, F.
  • Spagnuolo, A.
  • Zanetti, L.
  • Branno, M.
  • Sordino, P.

Abstract
    Historically, mutations have had a significant impact on the study of developmental processes and phenotypic evolution. Lesions in DNA are created by artificial methods or detected by natural genetic variation. Random mutations are then ascribed to genetic change by direct sequencing or positional cloning. Tunicate species of the ascidian genus Ciona represent nearly fully realized model systems in which gene function can be investigated in depth. Additionally, tunicates are valuable organisms for the study of naturally occurring mutations due to the capability to exploit genetic variation down to the molecular level. Here, we summarize the available information about how mutations are studied in ascidians with examples of insights that have resulted from these applications. We also describe notions and methodologies that might be useful for the implementation of easy and tight procedures for mutations studies in Ciona.

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