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Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues
Catarino, A.I.; Thompson, R.; Sanderson, W.; Henry, T.B. (2017). Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues. Environ. Toxicol. Chem. 36(4): 947-951. https://dx.doi.org/10.1002/etc.3608
In: Environmental Toxicology and Chemistry. Setac Press: New York. ISSN 0730-7268; e-ISSN 1552-8618, more
Peer reviewed article  
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Keyword
    Marine/Coastal
Authors  Top 
  • Catarino, A.I., more
  • Thompson, R.
  • Sanderson, W.
  • Henry, T.B.
Abstract
    The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH and enzyme digestions. The authors recommend use of industrial enzymes based on digestion efficiency, microplastic recovery, and avoidance of caustic chemicals.
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