Skip to main content
Publications | Persons | Institutes | Projects
[ report an error in this record ]basket (1): add | show Print this page

one publication added to basket [363503]
Saponin and fatty acid profiling of the sea cucumber Holothuria atra, α-glucosidase inhibitory activity and the identification of a novel triterpene-glycoside
Puspitasari, Y.E.; Tuenter, E.; Foubert, K.; Herawati, H.; Hariati, A.M.; Aulanni'am, A.; Pieters, L.; De Bruyne, T.; Hermans, N. (2023). Saponin and fatty acid profiling of the sea cucumber Holothuria atra, α-glucosidase inhibitory activity and the identification of a novel triterpene-glycoside. Nutrients 15(4): 1033. https://dx.doi.org/10.3390/nu15041033
In: Nutrients. MDPI AG: Basel. e-ISSN 2072-6643, more
Peer reviewed article  

Available in  Authors 

Keywords
    Holothuria Linnaeus, 1767 [WoRMS]; Holothuroidea [WoRMS]
    Marine/Coastal
Author keywords
    Holothuroidea; triterpene glycosides; Holothuria; a-glucosidase

Authors  Top 
  • Puspitasari, Y.E., more
  • Tuenter, E., more
  • Foubert, K., more
  • Herawati, H.
  • Hariati, A.M.
  • Aulanni'am, A.

Abstract
    Saponin-rich sea cucumber extracts have shown antidiabetic effects in a few reports. Although the triterpene glycosides of sea cucumbers are commonly isolated from their Cuvierian tubules, these are absent in Holothuria atra Jaeger. Therefore, this study intended to investigate the saponin profile in the body wall of H. atra, as well as to assess the α-glucosidase inhibitory activity of the H. atra extracts. The chemical profiling of sea cucumber extracts was conducted by UPLC-HRMS analysis. This resulted in the tentative identification of 11 compounds, 7 of which have not been reported in the H. atra body wall before. Additionally, two triterpene glycosides were purified and their structures were elucidated based on HRMS and NMR data: desholothurin B (1), and a novel epimer, 12-epi-desholothurin B (2). Moreover, the fatty acid profile of the H. atra body wall was investigated by GC-MS. It was found that the Me90 fraction of the H. atra body wall showed the strongest α-glucosidase inhibitory activity (IC50 value 0.158 ± 0.002 mg/mL), thus making it more potent than acarbose (IC50 value 2.340 ± 0.044 mg/mL).

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors