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y-glutamyl transpeptidase as a possible marker of sertoli cells in testes of fish
Kinnberg, K.; Christiansen, T.; Bjerregaard, P.; Korsgaard, B. (2000). y-glutamyl transpeptidase as a possible marker of sertoli cells in testes of fish, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 387
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more

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Keyword
    Marine/Coastal

Authors  Top 
  • Kinnberg, K.
  • Christiansen, T., more
  • Bjerregaard, P.
  • Korsgaard, B.

Abstract
    Finding markers directly correlating effects of estrogenic chemicals with the reproductive system of male fish is of great importance. In fish, gamma -glutamyl transpeptidase ( gamma -GTP) has been localised in various tissues, but not in testes. However, in testes of mammals gamma -GTP has been localised to Sertoli cells and used as a Sertoli cell marker. If this also applies to fish, gamma -GTP could be used as a marker of effects on Sertoli cells for studies of estrogenic chemicals. In our earlier studies nonylphenol treatment of eelpout resulted in a marked reduction in testicular gamma -GTP activity concomitant with a reduction in Sertoli cell size. In contrast, treatment of platyfish resulted in a tendency of increased gamma -GTP activity concomitant with a hypertrophy of Sertoli cells. To study our hypothesis of testicular gamma -GTP being a Sertoli cell marker we have examined the relationship between the histological structure of Sertoli cells and gamma -GTP activity in 6 fish species.

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