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Isolation of clonal cultures of endosymbiotic green algae from their ciliate hosts
Achilles-Day, U.E.M.; Day, J.G. (2013). Isolation of clonal cultures of endosymbiotic green algae from their ciliate hosts. J. microbiol. methods 92(3): 355-357. https://dx.doi.org/10.1016/j.mimet.2013.01.007
In: Journal of Microbiological Methods. Elsevier: Amsterdam. ISSN 0167-7012; e-ISSN 1872-8359, more
Peer reviewed article  
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Keywords
    Exploitable Scientific Result
    Marine Sciences
    Marine Sciences > Marine Sciences General
    Maritime Industries > Blue Biotech
    Scientific Community
    Scientific Publication
    Marine/Coastal
Author keywords
    Algal endosymbiosis; Paramecium bursaria; Euplotes daidaleos; Loxodesrostrum; Micromanipulation
Project Top | Authors 
  • Association of European marine biological laboratories, more
Authors  Top 
  • Achilles-Day, U.E.M.
  • Day, J.G.
Abstract
    Using Paramecium bursaria as a model organism improved protocols have been developed to isolate clonal endosymbiotic algae. This involved micromanipulation of individual protists, rupturing to release endosymbionts followed by enrichment on complex media and a series of plating steps, under low light (PAR ~ 10 μmol photons m− 2 s− 1).
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