A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals
Brenez, C.; Gerkens, P.; Mazzucchelli, G.; Jauniaux, T.; Eppe, G.; De Pauw, E.; De Pauw-Gillet, M.-C. (2004). A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals. Talanta 63(5): 1225-1230. https://hdl.handle.net/10.1016/j.talanta.2004.05.048
In: Talanta. Elsevier: Amsterdam. ISSN 0039-9140; e-ISSN 1873-3573, more
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| Keyword |
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| Author keywords |
PCDD/F mixture; seal PBMC; lymphoproliferation; proteomics |
| Authors | | Top |
- Brenez, C.
- Gerkens, P.
- Mazzucchelli, G., more
- Jauniaux, T., more
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- Eppe, G., more
- De Pauw, E., more
- De Pauw-Gillet, M.-C.
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| Abstract |
The cell lines chosen have demonstrated a positive response in terms of cell proliferation and associated modifications in proteins content, evaluated through DNA and proteins synthesis, at environmentally relevant dose of dioxins, brought by a typical environmental PCDD/F mixture. The response is time and species dependent. After completion of the identification of proteins affected by the intoxication, we will identify a set of specific proteins whose expression is correlated to the dioxin dose and submit the cell culture to the treatment with a single chemical as well as with mixtures. We hope that this will allow us to construct and validate a set of protein biomarkers of exposure to pollutants that will show a predictive aspect for unknown chemicals. The quantitative analysis of the set of biomarkers can then be a more specific bioassay and an alternative to physico-chemical or other already established bioanalytical methods for screening purposes. |
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