Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains
Lopez-Goni, I.; Garcia-Yoldi, D.; Marin, C.M.; de Miguel, M.J.; Munoz, P.M.; Blasco, J.M.; Jacques, I.; Grayon, M.; Cloeckaert, A.; Ferreira, A.C.; Cardoso, R.; Corrêa de Sa, M.I.; Walravens, K.; Albert, D.; Garin-Bastuji, B. (2008). Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. Journal of Clinical Microbiology 46(10): 3484-3487. https://dx.doi.org/10.1128/JCM.00837-08
In: Journal of Clinical Microbiology. AMER SOC MICROBIOLOGY: Washington. ISSN 0095-1137; e-ISSN 1098-660X, more
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| Authors | | Top |
- Lopez-Goni, I.
- Garcia-Yoldi, D.
- Marin, C.M.
- de Miguel, M.J.
- Munoz, P.M.
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- Blasco, J.M.
- Jacques, I.
- Grayon, M.
- Cloeckaert, A.
- Ferreira, A.C.
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- Cardoso, R.
- Corrêa de Sa, M.I.
- Walravens, K.
- Albert, D.
- Garin-Bastuji, B.
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| Abstract |
An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains. |
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