Influence of storage temperature on the stability of heat treated phycocyanin-λ-carrageenan complexes in liquid formulations
Buecker, S.; Grossmann, L.; Loeffler, M.; Leeb, E.; Weiss, J. (2022). Influence of storage temperature on the stability of heat treated phycocyanin-λ-carrageenan complexes in liquid formulations. Green Chem. 24(10): 4174-4185. https://dx.doi.org/10.1039/d2gc00809b
In: Green Chemistry. Royal Society of Chemistry: Cambridge. ISSN 1463-9262; e-ISSN 1463-9270, more
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| Authors | | Top |
- Buecker, S.
- Grossmann, L.
- Loeffler, M., more
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| Abstract |
The protein chromophore complex present in cyanobacteria like Arthrospira platensis can be utilized as a natural food colorant, but the appearance is prone to changes under environmental conditions that alter the protein-chromophore interactions. The study investigates the color stability of phycocyanin stabilized by λ-carrageenan during storage at 4 and 25 °C under different heating and pH conditions. Phycocyanin solutions and combinations with λ-carrageenan (1 : 4) were prepared at a pH of 2.5–6.0, with and without thermal treatment at 70 °C and 90 °C. Visual appearance, absorption spectra, zeta potential, and the diameter of the structures formed were observed over a period of 28 days. Considerable color losses were determined for pure phycocyanin at a pH of 2.5 to 4.5 when the samples were stored at 25 °C, which was improved by the formation of soluble complexes with λ-carrageenan in associative conditions. Interestingly, soluble complexes at a pH < pI were found to be more resistant at high treatment temperatures with a higher storage stability than co-soluble polymers at a pH > pI, but soluble structures formed at a pH of 2.5 had a significantly larger diameter (871 ± 185 nm) that increased in size during storage (2025 ± 1012 nm after 14 d at 25 °C) than at a pH of 3.0 (200 ± 5 nm) when heated to 90 °C. These results were related to changes in protein structure and mass transport phenomena and might be of high importance for low-viscous food formulations. |
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