Welkom op het expertplatform!
Dit platform verschaft informatie en kennis omtrent de WL expertisedomeinen 'hydraulica en sediment', 'havens en waterwegen', 'waterbouwkundige constructies', 'waterbeheer' en 'kustbescherming' - gaande van WL medewerkers met hun expertise, het curriculum van deze instelling, tot publicaties, projecten, data (op termijn) en evenementen waarin het WL betrokken is.
Het WL onderschrijft het belang van "open access" voor de ontsluiting van haar onderzoeksresultaten. Lees er meer over in ons openaccessbeleid.
| one publication added to basket [310371] |
High doses of sodium ascorbate act as a prooxidant and protect gnotobiotic brine shrimp larvae (Artemia franciscana) against Vibrio harveyi infection coinciding with heat shock protein 70 activation
Han, B.; Kaur, V.I.; Baruah, K.; Nguyen, D.V.; Bossier, P. (2019). High doses of sodium ascorbate act as a prooxidant and protect gnotobiotic brine shrimp larvae (Artemia franciscana) against Vibrio harveyi infection coinciding with heat shock protein 70 activation. Dev. Comp. Immunol. 92: 69-76. https://dx.doi.org/10.1016/j.dci.2018.11.007
In: Developmental and Comparative Immunology. Elsevier: New York,. ISSN 0145-305X; e-ISSN 0145-305X
|  |
| Trefwoord |
Artemia franciscana Kellog, 1906 [WoRMS]
|
| Author keywords |
Gnotobiotic brine shrimp; Sodium ascorbate; Prooxidant; Hydrogenperoxide; Heat shock protein 70 |
| Auteurs | | Top |
- Han, B.
- Kaur, V.I.
- Baruah, K.
|
|
|
| Abstract |
Ascorbate is an essential nutrient commonly regarded as an antioxidant. In this study, using axenic brine shrimp and pathogenic strain Vibrio harveyi as the host-pathogen model, we confirmed that pretreatment of sodium ascorbate (NaAs), at an optimum concentration, was a prooxidant by generation of hydrogen peroxide, inducing protective effects in the brine shrimp against V. harveyi infection. Such a protective effect could be neutralized by the addition of an antioxidant enzyme catalase. We further showed that generation of oxygen radicals is linked to the induction of heat shock protein 70 (Hsp70), which is involved in eliciting the antioxidant protection system including superoxidase dismutase (SOD) and possibly many other immune responses. Furthermore, using RNA interferencetechnique, we found that the pretreatment of sodium ascorbate increased the survival significantly in the control knockdown groups (using green fluorescent protein, GFP) but not in Hsp70 knockdown groups and the result directly suggested that the up-regulated Hsp70 induced by sodium ascorbate pretreatment induced the protective effect. These results provide a mechanistic rationale for exploring the further use of ascorbate for antimicrobial therapy in aquaculture. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
