Welkom op het expertplatform!
Dit platform verschaft informatie en kennis omtrent de WL expertisedomeinen 'hydraulica en sediment', 'havens en waterwegen', 'waterbouwkundige constructies', 'waterbeheer' en 'kustbescherming' - gaande van WL medewerkers met hun expertise, het curriculum van deze instelling, tot publicaties, projecten, data (op termijn) en evenementen waarin het WL betrokken is.
Het WL onderschrijft het belang van "open access" voor de ontsluiting van haar onderzoeksresultaten. Lees er meer over in ons openaccessbeleid.
Expression of virulence genes in luminescent and nonluminescent isogenic vibrios and virulence towards gnotobiotic brine shrimp (Artemia franciscana)
Ruwandeepika, H.; Defoirdt, T.; Bhowmick, P.; Karunasagar, I.; Bossier, P. (2011). Expression of virulence genes in luminescent and nonluminescent isogenic vibrios and virulence towards gnotobiotic brine shrimp (Artemia franciscana). J. Appl. Microbiol. 110(2): 399-406. dx.doi.org/10.1111/j.1365-2672.2010.04892.x
In: Journal of Applied Microbiology. Blackwell Science: Oxford. ISSN 1364-5072; e-ISSN 1365-2672
|   |
| Author keywords |
gene expression; quorum sensing; virulence |
| Auteurs | | Top |
- Ruwandeepika, H.
- Defoirdt, T.
- Bhowmick, P.
|
- Karunasagar, I.
- Bossier, P.
|
|
| Abstract |
Aims: This study aimed to evaluate the expression levels of virulence gene regulators (luxR and toxR) and virulence factors (serine protease, metalloprotease and haemolysin) in luminescent and nonluminescent isogenic Vibrio harveyi and Vibrio campbellii.Method and Results: Nonluminescent variants have been reported before to become dominant in cultures of luminescent vibrios when grown under static conditions in the dark. Wild-type V. harveyi BB120, V. campbellii LMG 21363, quorum sensing mutants of V. harveyi BB120 and their previously reported nonluminescent isogenic counterparts were used in this study. The expression level of the virulence genes srp serine protease, vhp metalloprotease and vhh haemolysin, the quorum sensing master regulator gene luxR and the virulence regulator gene toxR in isogenic luminescent and nonluminescent strains were quantified using reverse transcriptase real-time PCR. These experiments revealed that the nonluminescent strains produced lower levels of the quorum sensing master regulator gene luxR and the vhp metalloprotease gene (which is known to be regulated by quorum sensing). Finally, challenge tests with gnotobiotic brine shrimp (Artemia franciscana) larvae revealed that the nonluminescent strains are less virulent than their luminescent isogenic counterparts.Conclusion: Nonluminescent variants of V. harveyi and V. campbellii strains produce lower levels of the quorum sensing master regulator gene luxR and the vhp metalloprotease gene and are less virulent to brine shrimp than their isogenic luminescent counterparts.Significance and Impact of the study: These results indicate that adaptation of luminescent vibrios to specific growth conditions that result in a dominant nonluminescent phenotype is accompanied by a decreased adaptation to a host environment because of altered virulence gene regulation. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
