Onderzoeksoutput

Welkom op het expertplatform!

Dit platform verschaft informatie en kennis omtrent de WL expertisedomeinen 'hydraulica en sediment', 'havens en waterwegen', 'waterbouwkundige constructies', 'waterbeheer' en 'kustbescherming' - gaande van WL medewerkers met hun expertise, het curriculum van deze instelling, tot publicaties, projecten, data (op termijn) en evenementen waarin het WL betrokken is.

Het WL onderschrijft het belang van "open access" voor de ontsluiting van haar onderzoeksresultaten. Lees er meer over in ons openaccessbeleid.

log in

• 
  People
• 
  Curriculum
• 
  Projects
• 
  Data sets
• 
  Publications
• 
  Events

[ meld een fout in dit record ]

mandje (0): toevoegen | toon

---

DNA identification of species of the Anopheles maculipennis complex and first record of An. daciae in Belgium Smitz, N.; De wolf, K.; Gheysen, A.; Deblauwe, I.; Meganck, K.; De Witte, J.; Schneider, A.; Verlé, I.; Dekoninck, W.; Gombeer, S.; Vanderheyden, A.; De Meyer, M.; Backeljau, T.; Müller, R.; Van Bortel, W. (2021). DNA identification of species of the Anopheles maculipennis complex and first record of An. daciae in Belgium. Medical and Veterinary Entomology 35(3): 442-450. https://hdl.handle.net/10.1111/mve.12519 In: Medical and Veterinary Entomology. Wiley-Blackwell: Hoboken. ISSN 0269-283X; e-ISSN 1365-2915

---

Beschikbaar in	Auteurs
VLIZ: Open access 366109 [ download pdf ]

---

Trefwoord

Anopheles maculipennis

Author keywords

Anopheles maculipennis subgroup, cytochrome oxidase I (COI), internal transcribed spacer 2 (ITS2), malaria vector, occurrence, restriction fragment length polymorphism (RFL

---

Auteurs		Top
Smitz, N. De wolf, K. Gheysen, A. Deblauwe, I. Meganck, K.	De Witte, J. Schneider, A. Verlé, I. Dekoninck, W. Gombeer, S.	Vanderheyden, A. De Meyer, M. Backeljau, T. Müller, R. Van Bortel, W.

---

Abstract

The present study aimed at identifying the members of the Anopheles maculipennis complex (Diptera: Culicidae) occurring in Belgium. Therefore, the second internal transcribed spacer of nuclear ribosomal DNA (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI) loci were sequenced in 175 and 111 specimens, respectively, collected between 2007 and 2019. In parallel, the suitability of two species‐diagnostic PCR‐RFLP assays was tested. The identified specimens included: An. maculipennis s.s. (N = 105), An. daciae (N = 62), An. atroparvus (N = 6) and An. messeae (N = 2). Each species was characterized by unique ITS2 haplotypes, whereas COI only supported the monophyly of An. atroparvus, a historical malaria vector in Belgium. Species identification results were further supported by unique PCR‐RFLP banding patterns. We report for the first time An. daciae in Belgium, where it was found to co‐occur with An. maculipennis s.s. The latter was the most prevalent in the collection studied (60%) and appears to have the widest distribution in Belgium. As in other studies, An. daciae and An. messeae appeared the most closely related species, up to the point that their species status remains debatable, while their ecological differences, including vector competences, need further study.

---

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid

Top | Auteurs

IMIS is ontwikkeld en wordt gehost door het VLIZ.