Oceans and seas are often perceived as the last wilderness on the planet. However, anthropogenic actions are already impacting these ecosystems, ranging from the coasts and the sea surface to the open ocean and the deep sea floor. Marine ecosystems are currently affected by multiple human activities, such as eutrophication, overfishing, the introduction of non-indigenous species, the contamination by hazardous chemicals and (micro)plastics, etc., in addition to climate change, leading to impaired environmental conditions. Evidence is growing that these changing environmental conditions have negative effects on the biodiversity and functioning of marine food webs. Due to their rapid responses to environmental variation, planktonic organisms are used as bio-indicators of ecosystem changes. With the need for better understanding the impact of a changing environment on zooplankton communities, zooplankton monitoring programs have been carried out in the marine environment globally since the early 20th century. Most zooplankton monitoring studies focus mainly on variability in biodiversity and biomass. However, this approach is hindered by challenges in the identification, which is time-consuming, complicated and requires biological expertise. A combination of new technologies and techniques, together with classical in situ and laboratory studies, could improve our understanding of such biodiversity patterns by assessing the health and physiology of marine plankton. In this thesis, we aimed to apply molecular methods to investigate spatiotemporal patterns in zooplankton dynamics, as well as to investigate the influence of environmental variation and stressors on these dynamics. |