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Visualisation of microalgal lipid bodies through electron microscopy
Verwee, E.; van de Walle, D.; De Bruyne, M.; Mienis, E.; Sekulic, M.; Chaerle, P.; Vyverman, W.; Foubert, I.; Dewettinck, K. (2024). Visualisation of microalgal lipid bodies through electron microscopy. J. Microsc. 293(2): 118-131. https://dx.doi.org/10.1111/jmi.13259
In: Journal of Microscopy. Blackwell: Oxford. ISSN 0022-2720; e-ISSN 1365-2818
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| Trefwoorden |
Nannochloropsis oculata (Droop) D.J.Hibberd, 1981 [WoRMS]; Phaeodactylum tricornutum Bohlin, 1897 [WoRMS]
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| Author keywords |
cryo-planing; electron microscopy; freeze-fracturing; lipid bodies; Nannochloropsis oculata; Phaeodactylum tricornutum |
| Auteurs | | Top |
- Verwee, E.
- van de Walle, D.
- De Bruyne, M.
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- Mienis, E.
- Sekulic, M.
- Chaerle, P.
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- Vyverman, W.
- Foubert, I.
- Dewettinck, K.
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| Abstract |
In this study, transmission electron microscopy (TEM) and cryo-scanning electron microscopy (cryo-SEM) were evaluated for their ability to detect lipid bodies in microalgae. To do so, Phaeodactylum tricornutum and Nannochloropsis oculata cells were harvested in both the mid-exponential and early stationary growth phase. Two different cryo-SEM cutting methods were compared: cryo-planing and freeze-fracturing. The results showed that, despite the longer preparation time, TEM visualisation preceded by cryo-immobilisation allows a clear detection of lipid bodies and is preferable to cryo-SEM. Using freeze-fracturing, lipid bodies were rarely detected. This was only feasible if crystalline layers in the internal structure, most likely related to sterol esters or di-saturated triacylglycerols, were revealed. Furthermore, lipid bodies could not be detected using cryo-planing. Cryo-SEM is also not the preferred technique to recognise other organelles besides lipid bodies, yet it did reveal chloroplasts in both species and filament-containing organelles in cryo-planed Nannochloropsis oculata samples.
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