| one publication added to basket [120810] | Poly-ß-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii
Halet, D.; Defoirdt, T.; Van Damme, P.; Vervaeren, H.; Forrez, I.; Van de Wiele, T.; Boon, N.; Sorgeloos, P.; Bossier, P.; Verstraete, W. (2007). Poly-ß-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii. FEMS Microbiol. Ecol. 60(3): 363-369
In: FEMS Microbiology Ecology. Federation of European Microbiological Societies: Amsterdam. ISSN 0168-6496; e-ISSN 1574-6941, meer
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| Auteurs | | Top |
- Halet, D.
- Defoirdt, T., meer
- Van Damme, P.
- Vervaeren, H., meer
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- Forrez, I.
- Van de Wiele, T., meer
- Boon, N., meer
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| Abstract |
A poly-β-hydroxybutyrate (PHB)-accumulating enrichment culture was obtained using activated sludge from a polyphosphate-accumulating reactor as inoculum. PHB accumulated by the enrichment culture significantly enhanced the survival of Artemia nauplii, infected with the virulent pathogen Vibrio campbellii LMG 21363. A strain was isolated from the enrichment culture, based on its ability to accumulate PHB, and 16S rRNA gene sequencing of the isolate revealed 99% sequence similarity to Brachymonas denitrificans AS-P1. The isolate, named PHB2, showed good PHB-accumulating activity (up to 32% of the cell dry weight). PHB accumulated by isolate PHB2 was able to protect Artemia completely from the V. campbellii strain. Our data indicate that PHB-accumulating bacteria, such as B. denitrificans PHB2, could be used as an an effective and economically interesting alternative strategy to control infections in aquaculture. |
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