Overslaan en naar de inhoud gaan
Publicaties | Personen | Instituten | Projecten
[ meld een fout in dit record ]mandje (1): toevoegen | toon Print deze pagina

one publication added to basket [281024]
Neuropeptidomic analysis of the brain and thoracic ganglion from the Jonah crab, Cancer borealis
Huybrechts, J.; Nusbaum, M.P.; Vanden Bosch, L.; Baggerman, G.; De Loof, A.; Schoofs, L. (2003). Neuropeptidomic analysis of the brain and thoracic ganglion from the Jonah crab, Cancer borealis. Biochem. Biophys. Res. Commun. 308(3): 535-544. https://dx.doi.org/10.1016/S0006-291X(03)01426-8
In: Biochemical and Biophysical Research Communications. ACADEMIC PRESS INC ELSEVIER SCIENCE: San Diego etc.. ISSN 0006-291X; e-ISSN 1090-2104, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoorden
    Cancer borealis Stimpson, 1859 [WoRMS]
    Marien/Kust
Author keywords
    Q-TOF; MALDI-TOF imaging; mass spectrometry; peptidomics; Cancerborealis; orcokinin; FMRFamide-related peptides; allatostatins

Auteurs  Top 
  • Huybrechts, J.
  • Nusbaum, M.P.
  • Vanden Bosch, L.
  • Baggerman, G.
  • De Loof, A.
  • Schoofs, L., meer

Abstract
    Mass spectrometric methods were applied to determine the peptidome of the brain and thoracic ganglion of the Jonah crab (Cancer borealis). Fractions obtained by high performance liquid chromatography were characterized using MALDI-TOF MS and ESI-Q-TOF MS/MS. In total, 28 peptides were identified within the molecular mass range 750–3000 Da. Comparison of the molecular masses obtained with MALDI-TOF MS with the calculated molecular masses of known crustacean peptides revealed the presence of at least nine allatostatins, three orcokinin precursor derived peptides, namely FDAFTTGFGHS, [Ala13]-orcokinin, and [Val13]-orcokinin, and two kinins, a tachykinin-related peptide and four FMRFamide-related peptides. Eight other peptides were de novo sequenced by collision induced dissociation on the Q-TOF system and yielded AYNRSFLRFamide, PELDHVFLRFamide or EPLDHVFLRFamide, APQRNFLRFamide, LNPFLRFamide, DVRTPALRLRFamide, and LRNLRFamide, which belong to the FMRFamide related peptide family, as well as NFDEIDRSGFA and NFDEIDRSSFGFV, which display high sequence similarity to peptide sequences within the orcokinin precursor of Orconectes limosus. Our paper is the first (neuro)peptidomic analysis of the crustacean nervous system.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs