Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia fransiscana
Nelis, H.J.; Merchie, G.; Lavens, P.; Sorgeloos, P.; De Leenheer, A.P. (1994). Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia fransiscana. Anal. Chem. 66(8): 1330-1333
In: Analytical chemistry. American Chemical Society: Washington. ISSN 0003-2700; e-ISSN 1520-6882, meer
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| Trefwoorden |
Artemia franciscana Kellog, 1906 [WoRMS]
Marien/Kust; Brak water |
| Auteurs | | Top |
- Nelis, H.J.
- Merchie, G.
- Lavens, P., meer
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- Sorgeloos, P., meer
- De Leenheer, A.P.
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| Abstract |
Two producers are described for the solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia . The first one is based on reversed-phase ion pair extraction on an octadecylsilica cartridge using tetrabutylammonium as the counterion, followed by elution with methanol. In the second procedure ascorbic acid 2-sulfate is retained on a DEAE silica cartridge and eluted with 0.1M sodium salicylate. Both approaches were used as part of a quantitative reversed-phase ion pair liquid chromatographic method with detection at 254nm. The anion exchange extraction procedure is superior to its ion pair counterpart in terms of recovery (88.7±3,5% versus 63.0±12.7%), reproducibility (CVs of 3.6-7.7% versus 6.3-18.7%), and simplicity. |
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