Overslaan en naar de inhoud gaan
Publicaties | Personen | Instituten | Projecten
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

A natural point mutation changes both target selectivity and mechanism of action of sea anemone toxins
Peigneur, S.; Béress, L.; Möller, C.; Marí, F.; Forssmann, W.-G.; Tytgat, J. (2012). A natural point mutation changes both target selectivity and mechanism of action of sea anemone toxins. FASEB J. 26(12): 5141-5151. http://dx.doi.org/10.1096/fj.12-218479
In: The FASEB Journal. Federation of American Societies for Experimental Biology: Bethesda, Md.. ISSN 0892-6638; e-ISSN 1530-6860, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoord
    Marien/Kust
Author keywords
    voltage-gated sodium channel; voltage-gated potassium channel; ion channel inhibitor; ion channel modulator

Auteurs  Top 
  • Peigneur, S., meer
  • Béress, L.
  • Möller, C.
  • Marí, F.
  • Forssmann, W.-G.
  • Tytgat, J., meer

Abstract
    APETx3, a novel peptide isolated from the sea anemone Anthopleura elegantissima, is a naturally occurring mutant from APETx1, only differing by a Thr to Pro substitution at position 3. APETx1 is believed to be a selective modulator of human ether-á-go-go related gene (hERG) potassium channels with a Kd of 34 nM. In this study, APETx1, 2, and 3 have been subjected to an electrophysiological screening on a wide range of 24 ion channels expressed in Xenopus laevis oocytes: 10 cloned voltage-gated sodium channels (NaV 1.2–NaV1.8, the insect channels DmNaV1, BgNaV1–1a, and the arachnid channel VdNaV1) and 14 cloned voltage-gated potassium channels (KV1.1–KV1.6, KV2.1, KV3.1, KV4.2, KV4.3, KV7.2, KV7.4, hERG, and the insect channel Shaker IR). Surprisingly, the Thr3Pro substitution results in a complete abolishment of APETx3 modulation on hERG channels and provides this toxin the ability to become a potent (EC50 276 nM) modulator of voltage-gated sodium channels (NaVs) because it slows down the inactivation of mammalian and insect NaV channels. Our study also shows that the homologous toxins APETx1 and APETx2 display promiscuous properties since they are also capable of recognizing NaV channels with IC50 values of 31 nM and 114 nM, respectively, causing an inhibition of the sodium conductance without affecting the inactivation. Our results provide new insights in key residues that allow these sea anemone toxins to recognize distinct ion channels with similar potency but with different modulatory effects. Furthermore, we describe for the first time the target promiscuity of a family of sea anemone toxins thus far believed to be highly selective.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs