Publicaties | Personen | Instituten | Projecten
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina
Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK)
Phuoc, L.H.; Hu, B.; Wille, M.; Hien, N.T.; Phuong, V.H.; Tinh, N.T.N.; Loc, N.H.; Sorgeloos, P.; Bossier, P. (2016). Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK). J. Fish Dis. 39(5): 555-564. dx.doi.org/10.1111/jfd.12388
In: Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne. ISSN 0140-7775; e-ISSN 1365-2761, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Penaeus monodon Fabricius, 1798 [WoRMS]; Penaeus vannamei Boone, 1931 [WoRMS]; Vibrio Pacini, 1854 [WoRMS]
Author keywords
    DnaK heat-shock proteins; heat-shock protein; Penaeus monodon; Penaeusvannamei; recombinant protein; Vibrio
Auteurs  Top 
  • Phuoc, L.H.
  • Hu, B., meer
  • Wille, M., meer
  • Hien, N.T.
  • Phuong, V.H.
  • Tinh, N.T.N., meer
  • Loc, N.H.
  • Sorgeloos, P., meer
  • Bossier, P., meer
Abstract
    This study was conducted to test the effect of DnaK on priming immune responses in Penaeid shrimp. Juvenile-specific pathogen-free (SPF) P. vannamei shrimp were injected with 0.05 μg recombinant DnaK. One hour post-DnaK priming, a non-lethal dose of Vibrio campbellii (105 CFU shrimp−1) was injected. Other treatments include only DnaK or V. campbellii injection or control with blank inocula. The haemolymph of three shrimp from each treatment was collected at 1.5, 6, 9 and 12 h post-DnaK priming (hpp). It was verified that injection with DnaK and V. campbellii challenge affected the transcription of 3 immune genes, transglutaminase-1 (TGase-1), prophenoloxidase-2 (proPO-2) and endogenous HSP70 (lvHSP70). In P. monodon, shrimp were first injected with DnaK at a dose of 10 μg shrimp−1 and one hour later with 106 CFU of V. harveyi (BB120) shrimp−1. Shrimp injected with DnaK showed a significant increase in proPO expression compared to the control (P < 0.05). Yet a double injection (DnaK and Vibrio) seemed to cause an antagonistic response at the level of expression, which was not equalled at the level of PO activity. Those results suggest that DnaK is able to modulate immune responses in P. vannamei and P. monodon.
Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs